A physical linkage between cystic fibrosis airway surface dehydration and Pseudomonas aeruginosa biofilms

TitleA physical linkage between cystic fibrosis airway surface dehydration and Pseudomonas aeruginosa biofilms
Publication TypeJournal Article
Year of Publication2006
AuthorsH Matsui, VE Wagner, DB Hill, UE Schwab, TD Rogers, B Button, RM Taylor II, R Superfine, M Rubinstein, BH Iglewski, and RC Boucher
JournalProceedings of the National Academy of Sciences of the United States of America
Volume103
Start Page18131
Issue48
Pagination18131 - 18131
Date Published11/2006
Abstract

A vexing problem in cystic fibrosis (CF) pathogenesis has been to explain the high prevalence of Pseudomonas aeruginosa biofilms in CF airways. We speculated that airway surface liquid (ASL) hyperabsorption generates a concentrated airway mucus that interacts with P. aeruginosa to promote biofilms. To model CF vs. normal airway infections, normal (2.5% solids) and CF-like concen-trated (8% solids) mucus were prepared, placed in flat chambers, and infected with an Ϸ5 ؋ 10 3 strain PAO1 P. aeruginosa. Although bacteria grew to 10 10 cfu/ml in both mucus concentrations, mac-rocolony formation was detected only in the CF-like (8% solids) mucus. Biophysical and functional measurements revealed that concentrated mucus exhibited properties that restrict bacterial motility and small molecule diffusion, resulting in high local bac-terial densities with high autoinducer concentrations. These prop-erties also rendered secondary forms of antimicrobial defense, e.g., lactoferrin, ineffective in preventing biofilm formation in a CF-like mucus environment. These data link airway surface liquid hypera-bsorption to the high incidence of P. aeruginosa biofilms in CF via changes in the hydration-dependent physical– chemical properties of mucus and suggest that the thickened mucus gel model will be useful to develop therapies of P. aeruginosa biofilms in CF airways.

DOI10.1073/pnas.0606428103
Short TitleProceedings of the National Academy of Sciences of the United States of America